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Warner Instruments open perfusion chamber insert (rc-37f)
Open Perfusion Chamber Insert (Rc 37f), supplied by Warner Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perfusion+insert+rc-37f/perfusion+insert/pmc11369499-96-4-2
Average 90 stars, based on 1 article reviews
open perfusion chamber insert (rc-37f) - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2
Article Snippet: To monitor changes in microtubule nucleation within the same cells during activation, sensitized cells were placed on a 35 mm glass-bottom dish (Cellvis, Mountain View, CA, USA; D35-14-1.5-N) coated with fibronectin, and a perfusion insert RC-37F (Warner Instruments, Hamden, CT, USA) was positioned within the dish.

Article Title: Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by ARF GTPase-activating protein GIT2.
Article Snippet: To monitor changes in microtubule nucleation within the same cells during activation, sensitized cells were placed on a 35 mm glassbottom dish (Cellvis, Mountain View, CA, USA; D35-14-1.5-N) coated with fibronectin, and a perfusion insert RC-37F (Warner Instruments, Hamden, CT, USA) was positioned within the dish.

Incubation:

Article Title: STIM1-directed reorganization of microtubules in activated mast cells.
Article Snippet: Twenty-four hours later, 100 ml of cell suspension at concentration 1.53 106 cells/ml was overlaid on 35-mm glass-bottom culture dishes (MatTek, Ashland, MA; Cat. No. P35G-1.514-C) precoated with fibronectin (see above), and cells were allowed to attach for 1 h at 37 ̊C. .. Perfusion insert for the 35-mm culture dish was at E V A N S L IB R A R Y , FL O R ID A IN ST IT U T E O F T E C H N O L O G Y on M arch 18, 2015 http://w w w .jim m unol.org/ D ow nloaded from inserted (Warner Instruments, Hamden, CT; model RC-37F), and cells were washed and subsequently incubated in RPMI medium for live cell imaging (RPMI 1640 without phenol red, riboflavin, folic acid, pyridoxal, Fe[NO3]3) supplemented with 20 mM HEPES. .. Cells were imaged on the Leica AM total internal reflection fluorescence (TIRF) MC (Leica Microsystems) at 37 ̊C.

Live Cell Imaging:

Article Title: STIM1-directed reorganization of microtubules in activated mast cells.
Article Snippet: Twenty-four hours later, 100 ml of cell suspension at concentration 1.53 106 cells/ml was overlaid on 35-mm glass-bottom culture dishes (MatTek, Ashland, MA; Cat. No. P35G-1.514-C) precoated with fibronectin (see above), and cells were allowed to attach for 1 h at 37 ̊C. .. Perfusion insert for the 35-mm culture dish was at E V A N S L IB R A R Y , FL O R ID A IN ST IT U T E O F T E C H N O L O G Y on M arch 18, 2015 http://w w w .jim m unol.org/ D ow nloaded from inserted (Warner Instruments, Hamden, CT; model RC-37F), and cells were washed and subsequently incubated in RPMI medium for live cell imaging (RPMI 1640 without phenol red, riboflavin, folic acid, pyridoxal, Fe[NO3]3) supplemented with 20 mM HEPES. .. Cells were imaged on the Leica AM total internal reflection fluorescence (TIRF) MC (Leica Microsystems) at 37 ̊C.



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